![]() ![]() On the Trans-Blot SD Semi-Dry Transfer Cell page click on the document tab below the picture of the system. Of protein may be transferred through the membrane and onto the filter paper below. Under the strong fieldsĭeveloped by this apparatus, transfers may not always be quantitative. Recommended to prevent excessive heating during the run. A current limit (3 mA/cm2 for large gels 5.5 mA/cm2 for mini gels) is Large gelsĬan be transferred for 30 minutes to 1 hour at 15–25 V. Transfer mini gels for 15–30 minutes at 10–15 V. Although the Bio-Rad and Novex instructions don't seem to be that different (once again, see below) I think your best bet would be to follow the Novex instructions since they have probably optimized conditions for their apparatus.įrom the Trans-Blot SD Semi-Dry Transfer Cell, Rev C Instruction Manual (Part 1703940) For most proteins, I usually transfer at 15 V for 25 - 30 min as recommended by Bio-Rad (see below). See the ordering information for Mini-PROTEAN ® TGX™ Precast Gels and Criterion™ TGX™ Precast Gels to select the best gel for your application.I occasionally use the Bio-Rad Trans-blot SD for my transfers. See how the Trans-Blot Turbo transfer system fits into the V3 Western Workflow™ to streamline your western blotting process. Use Bio-Rad’s Precision Plus Protein™ WesternC™ Standards and Clarity™ Western ECL Substrate for chemiluminescence detection on both film and digital imagers. Select the Trans-Blot Turbo RTA transfer kit for a low-fluorescence membrane option. Trans-Blot Turbo transfer packs come with either PVDF or nitrocellulose membranes: Protein-protein or protein-ligand interactions.Evaluation of protein expression levels. ![]()
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